Coompo Research Chemicals

While the general toxicity of pachypodol was determined by the brine shrimp lethality assay, the cytotoxic potential of this flavonoid has been evaluated by the Promega's CellTiter 96 Non-Radioactive Cell Proliferation Assay using the CaCo-2 colon cancer cell line (IC50 = 185.6 µM). A summary of the biological activities of pachypodol reported to date is also presented.

Pachypodol was the most active compound on ATP synthesis inhibition. The I50 value was 51 μM. Pachypodol behaves as a Hill reaction inhibitor. It inhibited the uncoupled electron flow on photosystem II partial reaction from water to dichlorophenol indophenol (DCPIP) and from water to sodium silicomolybdate. However, the uncoupled partial reaction from diphenylcarbazide to DCPIP and the uncoupled photosystem I from DCPIPred to MV were not inhibited by Pachypodol. These results were corroborated by fluorescence decay data. Therefore, pachypodol inhibits the water-splitting enzyme activity.