Ethidium Bromide

Catalogue number C230017
Chemical nameEthidium Bromide
CAS Number1239-45-8
Synonyms3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide; 2,7-Diamino-10-ethyl-9- phenylphenanthridinium Bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium Bromide; 2,7-Diamino-9-phenylphenanthridine Ethobromide; Dromilac; Homidium Bromide;
Molecular WeightC21H20BrN3
Formula394.31
Purity98%
Physical DescriptionVery Dark Red to Dark Purple S
SolventChloroform, Dichloromethane,DMSO
StorageStored at 2-8°C, Protected from air and light, refrigerate or freeze
ApplicationsEthidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).
ReferencesWaring, M.J., et al.: J. Mol. Biol., 13, 269 (1965), Probst, G.S., et al.: Environ. Mutagen., 3, 11 (1981),
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Ethidium Bromide
Price(USD)
SizePrice(USD)Discount
5g$125.0010% OFF
25g$525.0010% OFF
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