Coompo Research Chemicals

Capillarisin (Cap) expressed a antioxidant property by its capacity for quenching the free radicals of 1,1-diphenyl-2-picrylhydrazyl (DPPH). This antioxidant bioactivity of Cap was investigated further using a model of t-butylhydroperoxide (t-BHP)-induced cytotoxicity and genotoxicity in rat primary hepatocytes. Results presented here demonstrate that Cap  significantly decreased the leakage of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) and the formation of malondialdehyde (MDA) induced by 30 min treatment of t-BHP (1.5 mM) in primary cultured rat hepatocytes. Cap also attenuated the t-BHP-induced diminution of glutathione (GSH) and high level of DNA repaired synthesis. These results lead to speculation that Cap presents inhibitory effects against t-BHP-caused cytotoxicity and genotoxicity in rat primary hepatocyte cultures at least via two distinct pathways, stabilizing the GSH system and quenching free radicals.

Cap induced penile relaxation and enhanced phosphodiesterase type 5 inhibitors -induced relaxation. Cap increased cGMP and cAMP in the perfusate. The application of Cap on penile corpus cavernosum pre-treated with L-NAME and ODQ significantly inhibited the relaxation. Cap exerts the relaxing effect on penile corpus cavernosum by activating the NO-cGMP and adenylyl cAMP signaling pathways and may become an alternative medicine for patients who want to use natural products to improve erectile function or do not completely respond to phosphodiesterase type 5 inhibitors.

Cap potentially inhibits the biomarkers related to inflammation through the abrogation of ERK, JNK, and NF-kappaB p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases.